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Aminoallyl-dUTP-ATTO-550

Aminoallyl-dUTP-ATTO550 is recommended for direct enzymatic labeling of DNA/cDNA by Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling.Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Recommended Aminoallyl-dUTP-ATTO550/dTTP ratio Nick Translation: 30-50% Aminoallyl-dUTP-ATTO550/ 50% dTTP

Product Specifications

UNSPSC

12352208

UNSPSC Description

Nucleic acids

HS Code

29349990

eClass Number

34160990

eClass Product Group

Nucleinsäure (nicht klassifiziert)

Applications

Incorporation into DNA/cDNA by - Nick Translation with DNAse I/ DNA Polymerase I in-house data

Concentration

(1 mM)

Purity

≥ 95 % (HPLC)

Form

Filtered solution (30 kDa) in 10 mM Tris-HCl

pH

7.5 ±0.5

Color

Red-violet

Autoclavability

false

Smiles

[C@@H]1 (C[C@@H] (O[C@@H]1COP (=O) (OP (=O) (OP (=O) ([O-]) O) O) O) n1c (=O) [nH]c (=O) c (c1) /C=C/CNC (=O) CCCN (C (=O) c1c (C2=c3cc4C (CC ([NH+]=c4cc3Oc3cc4NC (CC (c4cc23) C) (C) C) (C) C) C) cccc1) C) O

Shipping Conditions

Shipped on gel packs

Storage Conditions

Store at -20 °C Short term exposure up to 1 week cumulative to ambient temperature possible.

Storage Temperature

Store at -20 °C

Notes

Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life

12 months after date of delivery

Product Datasheet

https://cdn.gentaur.com/products/220/68dd094d061270361e3dc8e2/datasheet/nu-803-550-s.pdf

Product MSDS

https://cdn.gentaur.com/products/220/68dd094d061270361e3dc8e2/msds/nu-803-550-s.pdf

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