Aminoallyl-dUTP-ATTO-425
Aminoallyl-dUTP-ATTO425 is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling.Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine.
Product Specifications
UNSPSC
12352208
UNSPSC Description
Nucleic acids
HS Code
29349990
eClass Number
34160990
eClass Product Group
Nucleinsäure (nicht klassifiziert)
Applications
Incorporation into DNA/cDNA by - PCR with Taq polymerase in-house data - Nick Translation with DNAse I/ DNA Polymerase I in-house data
Concentration
(1 mM)
Purity
≥ 95 % (HPLC)
Form
Solution in water
pH
7.5 ±0.5
Color
Green-yellow
Autoclavability
false
Smiles
O=C(OCC)c1cc2c(oc1=O)cc1N(CCCC(=O)NC/C=C/c3cn(c(=O)[nH]c3=O)[C@H]3C[C@@H]([C@H](O3)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)C(C)(C)CC(C)c1c2
Shipping Conditions
Shipped on gel packs
Storage Conditions
Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Storage Temperature
Store at -20 °C
Notes
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life
12 months after date of delivery
Frequently Asked Questions
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