PCp-Biotin
pCp-Biotin is recommended for 3'-End Labeling of RNA/miRNA with T4 RNA Ligase that conjugates a single Biotin molecule to the 3'-OH group of the RNA/miRNA template. The resulting Biotin-labeled RNA/miRNA probes are ideally suited for hybridization experiments such as microarray-based expression profiling or electro mobility shift assays (EMSA). Detection of the Biotin moiety can be performed using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP) or a fluorescent dye. Alternatively, Biotin-labeled RNA/miRNA probes can also be purified with Streptavidin-conjugated agarose/magnetic beads.
Product Specifications
UNSPSC
12352208
UNSPSC Description
Nucleic acids
HS Code
29349990
eClass Number
34160990
eClass Product Group
Nucleinsäure (nicht klassifiziert)
Applications
Incorporation into RNA/miRNA by 3'-End Labeling with T4 RNA Ligase
Concentration
(1 mM)
Purity
≥ 95 % (HPLC)
Form
Solution in 10 mM Tris-HCl
pH
7.5 ±0.5
Color
Colorless to slightly yellow
Autoclavability
false
Smiles
Nc1nc (=O) n (cc1) [C@@H]1O[C@H] (COP (=O) (O) O) [C@H] ([C@H]1O) OP (=O) (OCCCCCCNC (=O) CCCC[C@@H]1SC[C@H]2[C@@H]1NC (=O) N2) O
Shipping Conditions
Shipped on gel packs
Storage Conditions
Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Storage Temperature
Store at -20 °C
Notes
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life
12 months after date of delivery
Product Datasheet
https://cdn.gentaur.com/products/220/68dd0950061270361e3dd9ec/datasheet/nu-1706-bio.pdf
Product MSDS
https://cdn.gentaur.com/products/220/68dd0950061270361e3dd9ec/msds/nu-1706-bio.pdf
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