Azide-PEG4-aminoallyl-dUTP
Azide-PEG4-aminoallyl-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR. It is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The resulting Azide-functionalized DNA/cDNA can subsequently be labeled via Cu(I)-catalyzed or Cu(I)-free click chemistry that offers the choice to introduce a Biotin group (via Alkynes of Biotin or DBCO-functionalized Biotin, respectively) for subsequent purification tasks to introduce fluorescent group (via Alkynes of fluorescent dyes or DBCO-functionalized fluorescent dyes, respectively) for subsequent microscopic imaging to crosslink the DNA to Azide- or DBCO-functionalized biomolecules e.g.proteins
Product Specifications
UNSPSC
12352208
UNSPSC Description
Nucleic acids
HS Code
29349990
eClass Number
34160990
eClass Product Group
Nucleinsäure (nicht klassifiziert)
Applications
Incorporation into DNA/cDNA by PCR with Taq polymerase in-house data 3'-End Labeling with Terminal deoxynucleotidyl Transferase (TdT)[1]
Concentration
(10 mM)
Purity
≥ 95 % (HPLC)
Form
Solution in water
pH
7.5 ± 0.5
Color
Colorless to slightly yellow
Autoclavability
false
Smiles
[C@@H]1(C[C@@H](O[C@@H]1COP(=O)(OP(=O)(OP(=O)(O)O)O)O)n1c(=O)[nH]c(=O)c(c1)/C=C/CNC(=O)CCOCCOCCOCCOCCN=[N+]=[N-])O
Shipping Conditions
Shipped on gel packs
Storage Conditions
Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Storage Temperature
Store at -20 °C
Notes
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life
12 months after date of delivery
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