5-Azido-C3-UTP

Incorporation into RNA by T7 RNA polymerase-mediated in vitro transcription (up to 1400 bp tested). For a comprehensive protocol please refer to PP-501 or PP-501-Cy3/Cy5. 3'-Azide-Labeling of T7 promotor-containing oligonucleotides[1,2,3] The resulting azide-functionalized RNA can subsequently be processed via Cu(I)-free (azide-DBCO) or Cu(I)-catalyzed (azide-alkyne) click chemistry that offers the choice - to introduce a Biotin group for subsequent purification tasks (via DBCO-functionalized Biotin or Alkynes of Biotin, respectively) - to introduce fluorescent group for subsequent microscopic imaging (DBCO-functionalized fluorescent dyes or Alkynes of fluorescent dyes, respectively) - to crosslink the RNA to azide- or alkyne functionalized biomolecules e.g.proteins