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Biotin-11-dATP

Biotin-11-dATP is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dATP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal substrate properties and thus labeling efficiency as well as an efficient detection of the Biotin moiety is ensured by a 11-atom linker attached to the 7-Deaza position of adenine.

Product Specifications

UNSPSC

12352208

UNSPSC Description

Nucleic acids

HS Code

29349990

eClass Number

34160990

eClass Product Group

Nucleinsäure (nicht klassifiziert)

Applications

Incorporation into DNA/cDNA by - PCR with Taq polymerase [1] & in-house data - Nick Translation with DNAse I/ DNA Polymerase I [2] - Primer Extension with Klenow exo- [3,4] - Reverse Transcription with MMLV Reverse Transcriptase [5]

Concentration

(1 mM)

Purity

≥ 95 % (HPLC)

Form

Filtered solution (30 kDa) in 10 mM Tris-HCl

pH

7.5 ±0.5

Color

Colorless to slightly yellow

Autoclavability

false

Smiles

N1C(=O)N[C@@H]2[C@H]1[C@H](CCCCC(=O)NCCCCCC(=O)NCC#Cc1cn(c3ncnc(N)c13)[C@@H]1O[C@H](COP(=O)(O)OP(=O)(O)OP(=O)(O)O)[C@@H](O)C1)SC2

Shipping Conditions

Shipped on gel packs

Storage Conditions

Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Storage Temperature

Store at -20 °C

Notes

Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life

12 months after date of delivery

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