N6-Propargyl-ATP (N6pATP)

in vitro AMPylation of proteins[1,2] in vitro polyadenylation of RNA[3] The resulting alkyne-functionalized protein[1,2] or RNA[3] can subsequently be processed via Cu(I)-catalyzed (azide-alkyne) click chemistry that offers the choice to introduce a Biotin group for subsequent purification tasks (via Azides of Biotin) to introduce fluorescent group for subsequent microscopic imaging (via Azides of fluorescent dyes) to crosslink the RNA to azide-functionalized biomolecules e.g.proteins Presolski et al.[4] and Hong et al.[5] provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays. Agonistic ligand, mainly for nucleoside receptor A1 Nucleoside-triphosphates can be converted by different membrane-bound phosphatases into nucleosides acting as nucleoside receptor ligands. In some cases nucleoside phosphates act also directly on nucleoside receptors.