5-Azidomethyl-2'-deoxyuridine (5-AmdU)
5-AmdU (5-Azidomethyl-2'-deoxyuridine) can be used as a replacement for BrdU (5-Bromo-2'-deoxyuridine) or the copper-catalyst requiring 5-EdU (5-Ethynyl-2'-deoxyuridine) to measure de novo DNA synthesis during the S-phase of the cell cycle. 5-AmdU is cell permeable and incorporates into peplicating DNA instead of its natural analog thymidine. The resulting azide-functionalized DNA can subsequently be detected via Cu(I)-catalyzed (CuAAC) or Cu(I)-free Click Chemistry that offers the choice to introduce a Biotin group for subsequent purification tasks (via Alkynes of Biotin or DBCO-functionalized Biotin, respectivly) introduce a fluorescent group for subsequent microscopic imaging (via Alkynes of fluorescent dyes or DBCO-functionalized fluorescent dyes, respectivly).
Product Specifications
UNSPSC
12352208
UNSPSC Description
Nucleic acids
HS Code
29349990
eClass Number
34160990
eClass Product Group
Nucleinsäure (nicht klassifiziert)
Applications
DNA synthesis monitoring[1]
Purity
≥ 90 % (HPLC)
Form
Solid
Color
White to off-White
Autoclavability
false
Solubility
DMSO, methanol
Smiles
O=c1c(cn(c(=O)[nH]1)[C@@H]1O[C@H](CO)[C@@H](O)C1)CN=[N+]=[N-]
Shipping Conditions
Shipped at ambient temperature
Storage Conditions
Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Storage Temperature
Store at -20 °C
Notes
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life
12 months after date of delivery
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