5-DBCO-PEG4-dUTP
5-DBCO-PEG4-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR. It is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The resulting DBCO-functionalized DNA/cDNA can subsequently be labeled via Cu(I)-free click chemistry that offers the choice to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks to introduce fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging to crosslink the DNA to Azide-functionalized biomolecules e.g.proteins
Product Specifications
UNSPSC
12352200
UNSPSC Description
Biochemicals
HS Code
29349990
eClass Number
32160405
eClass Product Group
Biochemikalie (Labor, Sonstige)
Applications
Incorporation into DNA/cDNA by PCR with Taq polymerase in-house data
Concentration
(10 mM)
Purity
≥ 95 % (HPLC)
Form
Solution in 100 mM Tris-HCl
pH
7.5 ±0.5
Color
Colorless to slightly yellow
Autoclavability
false
Smiles
C1(=O)[nH]c(=O)n(cc1/C=C/CNC(=O)CCOCCOCCOCCOCCNC(=O)CCC(=O)N1Cc2c(C#Cc3c1cccc3)cccc2)[C@H]1C[C@H](O)[C@H](O1)COP(=O)(O)OP(=O)(O)OP(=O)(O)O
Shipping Conditions
Shipped on gel packs
Storage Conditions
Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Storage Temperature
Store at -20 °C
Notes
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life
12 months after date of delivery
Frequently Asked Questions
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