Anti-Pam-Igloo-L

The specificity against P-IGL-L, but not P-IGL-S, was demonstrated by transfected CHO cells, where the antibody selectively recognizes IGL-L. This P-IGL-L-like immunoreactivity (P-IGL-L-li) was colocalized with the fluorescence signal from the IGL-L-EGFP fusion protein. Furthermore, the antiserum was tested on neuronal tissue sections of the American cockroach: P-IGL-L-li could be blocked by preincubation of the antibody with recombinant P-IGL-L protein. There was also no immunostaining after replacement of antiserum with preimmune serum (Fig 2). The specificity of the antiserum against Periplaneta americana IGL-L was investigated by immunostaining of brain sections from different cockroach species.