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IgG Fraction of anti-Rabbit IgG [H&L] [Goat]

Product Specifications

CAS Number

9007-83-4

Reactivity

Rabbit

Immunogen

Rabbit IgG whole molecule

Type

Antibodies-Secondary

Applications

Suitable for immunoprecipitation, immunodiffusion, conjugation and most immunological methods requiring high titer and specificity.

Concentration

10.0 mg/ml (by UV absorbance at 280 nm)

Purity

This product is an IgG fraction purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum.

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Shipping Conditions

Ice Pack

Storage Conditions

Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of restoration.

Applications Notes

ELISA 1:70,000 - 1:300,000 WESTERN BLOT 1:5,000 - 1:25,000 IMMUNOHISTOCHEMISTRY 1:1,000 - 1:5,000 OTHER APPLICATIONS User Optimized

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