Fluorescein Conjugated F(ab)2 fragment anti-Goat IgG [H&L] [Mouse]
Selected Variant
SG009
Size: 5 mg
Product Specifications
CAS Number
9007-83-4
Reactivity
Goat
Immunogen
Goat IgG whole molecule
Type
Antibodies-Secondary
Applications
Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other based fluorescent assays requiring the absence of F(c) mediated binding and greater permeability.
Concentration
10.0 mg/ml (by UV absorbance at 280 nm)
Purity
This product is a F(ab)2 fragment of an IgG fraction purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum, Goat IgG and Goat Serum. No reaction was observed against anti-Mouse IgG F(c) or anti-Pepsin.
Buffer
0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.4
Shipping Conditions
Ice Pack
Storage Conditions
<p>Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of restoration.</p>
Applications Notes
Not specified. Optimal titers for applications should be determined by the researcher
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