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Peroxidase Conjugated Affinity Purified anti-Human IgG [H&L] [Rabbit]

Minimum Cross reactivity to Mouse Serum Proteins

Product Specifications

CAS Number

9007-83-4

Reactivity

Human

Immunogen

Human IgG whole molecule

Type

Antibodies-Secondary

Applications

Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase- based enzymatic assays requiring lot-to-lot consistency.

Concentration

2.0 mg/ml (by UV absorbance at 280 nm)

Purity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Human IgG and Human Serum. No reaction was observed against Mouse Serum Proteins.

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Shipping Conditions

Ice Pack

Storage Conditions

This product is stable for several weeks at 4° C as an undiluted liquid. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.

Applications Notes

ELISA 1:180,000 WESTERN BLOT 1:2,000 - 1:10,000 IMMUNOHISTOCHEMISTRY 1:1,000 - 1:5,000 OTHER APPLICATIONS User Optimized

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