Welcome to GenPrice! Check out our latest updates.

Shopping Cart (0)

Your cart is empty

Add some products to get started!

Alkaline Phosphatase Conjugated Affinity Purified anti-Human IgG [H&L] [Donkey]

Minimum Cross reactivity to Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Mouse, Rabbit, Rat and Sheep Serum Proteins

Product Specifications

CAS Number

9007-83-4

Reactivity

Human

Immunogen

Human IgG whole molecule

Type

Antibodies-Secondary

Applications

Suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase- based enzymatic assays requiring lot-to-lot consistency.

Concentration

1.0 mg/ml (by UV absorbance at 280 nm)

Purity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Donkey Serum, Human IgG and Human Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Mouse, Rabbit, Rat and Sheep Serum Proteins.

Buffer

0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0

Shipping Conditions

Ice Pack

Storage Conditions

Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of opening product.

Applications Notes

ELISA 1:2,000 - 1:10,000 WESTERN BLOT 1:500 - 1:2,500 IMMUNOHISTOCHEMISTRY 1:200 - 1:1,000

Frequently Asked Questions

More Discoveries

Explore Other Products

Browse additional items from our catalog

HSP60 (LK1), CF594 conjugate, 0.1mg/mL
BNC940851-500 1x 500 µL

HSP60 (LK1), CF594 conjugate, 0.1mg/mL

Sign In for Pricing
View Details
Cytokeratin 7/17 (C-46), CF640R conjugate, 0.1mg/mL
BNC400949-100 1x 100 µL

Cytokeratin 7/17 (C-46), CF640R conjugate, 0.1mg/mL

Sign In for Pricing
View Details
CD171 / L1CAM (L1 Cell Adhesion Molecule) (UJ127), CF405S conjugate, 0.1mg/mL
BNC040679-100 1x 100 µL

CD171 / L1CAM (L1 Cell Adhesion Molecule) (UJ127), CF405S conjugate, 0.1mg/mL

Sign In for Pricing
View Details
CD8A (Cytotoxic- & Suppressor T-Cell Marker) (C8/1779R), CF405S conjugate, 0.1mg/mL
BNC041779-100 1x 100 µL

CD8A (Cytotoxic- & Suppressor T-Cell Marker) (C8/1779R), CF405S conjugate, 0.1mg/mL

Sign In for Pricing
View Details
CD68 (KP1), CF555 conjugate, 0.1mg/mL
BNC550512-500 1x 500 µL

CD68 (KP1), CF555 conjugate, 0.1mg/mL

Sign In for Pricing
View Details
Cytokeratin 8 (KRT8) (rB22.1), CF647 conjugate, 0.1mg/mL
BNC472175-500 1x 500 µL

Cytokeratin 8 (KRT8) (rB22.1), CF647 conjugate, 0.1mg/mL

Sign In for Pricing
View Details