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Alkaline Phosphatase Conjugated Affinity Purified anti-Swine IgG (H&L) [Goat]

Selected Variant

SA031

Size: 250 µg

Product Specifications

CAS Number

9007-83-4

Reactivity

Swine

Immunogen

Swine IgG whole molecule

Type

Antibodies-Secondary

Applications

Suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase- based enzymatic assays requiring lot-to-lot consistency.

Concentration

1.0 mg/ml (by UV absorbance at 280 nm)

Purity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Swine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Swine IgG and Swine Serum.

Buffer

0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0

Shipping Conditions

Ice Pack

Storage Conditions

Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of opening product.

Applications Notes

ELISA 1:2,000 - 1:10,000 WESTERN BLOT 1:500 - 1:2,500 IMMUNOHISTOCHEMISTRY 1:200 - 1:1,000

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