Fluorescein Conjugated Affinity Purified anti-Human IgG (H&L) [Rabbit]
Minimum Cross-Reactivity to Mouse Serum Proteins
Product Specifications
CAS Number
9007-83-4
Reactivity
Human
Immunogen
Human IgG whole molecule
Type
Antibodies-Secondary
Applications
Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other based fluorescent assays requiring lot-to-lot consistency.
Concentration
2.0 mg/ml (by UV absorbance at 280 nm)
Purity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Human IgG and Human Serum. No reaction was observed against Mouse Serum Proteins.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Shipping Conditions
Ice Pack
Storage Conditions
This product is stable for several weeks at 4° C as an undiluted liquid. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.
Applications Notes
FLOW CYTOMETRY 1:2,000 - 1:10,000 IF MICROSCOPY 1:500 - 1:2,500 OTHER APPLICATIONS User Optimized
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