Texas Red Conjugated Affinity Purified anti-Rat IgG [H&L] [Donkey]

Minimum Cross Reactivity to Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins

Product Specifications

Reactivity

Rat

Immunogen

Rat IgG whole molecule

Type

Antibodies-Secondary

Applications

Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other based fluorescent assays requiring lot-to-lot consistency.

Concentration

1.0 mg/ml (by UV absorbance at 280 nm)

Purity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Rat IgG and Rat Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins.

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Shipping Conditions

Ice Pack

Storage Conditions

For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.