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Cy2 Conjugated Affinity Purified anti-Human IgG [H&L] [Donkey]

Minimum Cross Reactivity to Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Mouse, Rabbit, Rat and Sheep Serum Proteins

Product Specifications

CAS Number

9007-83-4

Reactivity

Human

Immunogen

Human IgG, whole molecule

Type

Antibodies-Secondary

Applications

Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other based fluorescent assays requiring lot-to-lot consistency.

Concentration

1.0 mg/ml

Purity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Human IgG and Human Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Mouse, Rabbit, Rat and Sheep Serum Proteins.

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Shipping Conditions

Ice Pack

Storage Conditions

For extended storage mix product with glycerol to 50% and then aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is three (3) months from date of restoration.

Applications Notes

FLOW CYTOMETRY 1:200 - 1:400 IF MICROSCOPY 1:100 - 1:1,000 OTHER APPLICATIONS User Optimized

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