Texas Red Conjugated Affinity Purified anti-Mouse IgG [H&L] [Donkey]
Minimum Cross Reactivity to Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins
Product Specifications
Reactivity
Mouse
Immunogen
Mouse IgG whole molecule
Type
Antibodies-Secondary
Applications
Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other based fluorescent assays requiring lot-to-lot consistency.
Concentration
1.0 mg/ml (by UV absorbance at 280 nm)
Purity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Mouse IgG and Mouse Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Shipping Conditions
Ice Pack
Storage Conditions
For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.
Applications Notes
Not specified. Optimal titers for applications should be determined by the researcher.
CAS Number
9007-83-4
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