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DyLight 549 Conjugated Affinity Purified anti-Rat IgG [H&L] [Goat]

Minimum Cross Reactivity to Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, and Sheep Serum Proteins

Product Specifications

CAS Number

9007-83-4

Reactivity

Rat

Immunogen

Rat IgG, whole molecule

Type

Antibodies-Secondary

Applications

WB, Immunofluorescence Microscopy

Concentration

1ug/ul

Purity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG and Rat Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins. This will react with heavy chains of rat IgG and with light chains of most rat immunoglobulins.

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Shipping Conditions

Ice Pack

Storage Conditions

This product is stable for several weeks at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use. Expiration date is one (1) year from date of receipt if properly stored.

Applications Notes

FLISA: >1:20,000. WB: >1:10,000. IF: >1:5,000. OTHER: User Optimized

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