Peroxidase Conjugated Affinity Purified anti-Mouse IgG (H&L) [Goat]
Product Specifications
CAS Number
9007-83-4
Reactivity
Mouse
Immunogen
Mouse IgG, whole molecule
Type
Antibodies-Secondary
Applications
Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase- based enzymatic assays requiring lot-to-lot consistency.
Concentration
2.0 ug/ul (by UV absorbance at 280 nm)
Purity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgG and Mouse Serum.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Shipping Conditions
Ice Pack
Storage Conditions
For extended storage, aliquot contents and
freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Expiration date is one (1) year after opening the vial.
Applications Notes
ELISA 1:100,000 WESTERN BLOT 1:2,000 - 1:10,000 IMMUNOHISTOCHEMISTRY 1:500 - 1:2,500
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