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Peroxidase Conjugated Affinity Purified anti-Mouse IgG (H&L) [Goat]

Selected Variant

SG023

Size: 1 mg

Product Specifications

CAS Number

9007-83-4

Reactivity

Mouse

Immunogen

Mouse IgG, whole molecule

Type

Antibodies-Secondary

Applications

Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase- based enzymatic assays requiring lot-to-lot consistency.

Concentration

2.0 ug/ul (by UV absorbance at 280 nm)

Purity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgG and Mouse Serum.

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Shipping Conditions

Ice Pack

Storage Conditions

For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Expiration date is one (1) year after opening the vial.

Applications Notes

ELISA 1:100,000 WESTERN BLOT 1:2,000 - 1:10,000 IMMUNOHISTOCHEMISTRY 1:500 - 1:2,500

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