Anti-4E-BP1(Phospho-Thr45)
Product Specifications
CAS Number
9007-83-4
Reactivity
Human,Mouse,Rat
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human 4E-BP1 around the phosphorylation site of threonine 45 (S-T-TP-P-G).
Clonality
Polyclonal
Type
Antibodies-Polyclonal
Applications
WB,IHC
Concentration
1ug/ul
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using
epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed
by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Buffer
phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Shipping Conditions
Ice Pack
Storage Conditions
This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Specificity
4E-BP1 (phospho-Thr45) antibody detects endogenous levels of 4E-BP1 only when
phosphorylated at threonine 45. This antibody may cross-react with 4E-BP2 and 4E-BP3
when phosphorylated at equivalent sites.
Applications Notes
WB: 1:500~1:1000 IHC: 1:50~1:100
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