Anti-ZCSL3
Diphthamide is a unique posttranslationally modified histidine found only in translation elongation factor-2 (EEF2; MIM 130610). This modification is conserved from archaebacteria to humans and serves as the target for ADP-ribosylation and inactivation of EEF2 by diphtheria toxin (DT) and Pseudomonas exotoxin A. DPH4 is 1 of several enzymes involved in synthesis of diphthamide in EEF2
Product Specifications
CAS Number
9007-83-4
Reactivity
Human,Mouse,Rat
Immunogen
a 16 amino acid peptide from the intermediate residues of human ZCSL3 protein.
Clonality
Polyclonal
Type
Antibodies-Polyclonal
Applications
WB,ELISA
Concentration
100ug/100ul
Purity
Affinity purified
Buffer
PBS, pH 7.4 with 0.02% Sodium Azide
Shipping Conditions
Ice Pack
Storage Conditions
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Fragment
Rabbit IgG
Specificity
Not yet tested in other species
Applications Notes
For research use only
Peptide ELISA titer: 1:20,000-1:80,000
Western blot analysis: 1:250-500
Optimal dilutions/concentrations should be determined by the end use.
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