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Anti-Saporin

Saporin is a ribosome-inactivating protein (RIP) of type I. This monomeric RNA N-glycosidase purified from seeds of the plant Saponaria officinalis also known as Soapwort, is capable of specific depurination of eukaryotic ribosomes thus arresting protein synthesis. No ligand has been identified in saporin hence its inability to transverse the cell membrane. Due to its toxicity and stability of the structure, saporin has proven extremely useful for construction of immunotoxins. The expected molecular weight of the purified saporin is 29.5 kDa.

Product Specifications

CAS Number

9007-83-4

Reactivity

Other

Immunogen

Saporin, whole molecule

Clonality

Polyclonal

Type

Antibodies-Polyclonal

Applications

WB,IHC,ELISA,ICC/IF

Concentration

1ug/ul

Purity

Filter purified

Buffer

PBS, pH 7.4 with 0.02% sodium azide.

Shipping Conditions

Ice Pack

Storage Conditions

This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Specificity

Confirmed to react with purified saporin.

Applications Notes

A dilution of 1:200 to 1: 2000 is recommended. The optimal working dilution should be determined by the end user.

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