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Anti-Monoclonal Anti-Phosphothreonine-Biotin produced in mouse

Monoclonal Anti-Phosphothreonine−Biotin antibody produced in mouse clone PTR-8, purified immunoglobulin, buffered aqueous solution

Product Specifications

CAS Number

9007-83-4

Reactivity

Human

Immunogen

phosphothreonine conjugated to keyhole limpet hemocyanin (KLH).

Clonality

Monoclonal

Clone

PTR-8

Type

Antibodies-Monoclonal

Applications

ELISA,Other

Concentration

1ug/ul

Purity

purified immunoglobulin

Buffer

PBS, pH 7.4 with 0.05% sodium azide.

Shipping Conditions

Ice Pack

Storage Conditions

This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Fragment

IgG2b

Specificity

As determined by ELISA and dot blot, the antibody reacts specifically with phosphorylated threonine, both as free amino acid or conjugated to carriers such as BSA or KLH. No cross-reactivity is observed with non-phosphorylated threonine, phosphoserine, phosphotyrosine, AMP or ATP. This antibody has been used in immunoblotting for the localization of some phosphothreonine-containing proteins. Certain proteins known to contain phosphorylated threonine may not be recognized by this antibody due to steric hindrance of the recognition site.

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