Anti-Human IL-6

This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

This antibody detects recombinant and native IL-6 present in body fluids and cell supernatants in various assays (i.e. IL-1 stimulated IL-6 production from fibroblasts). In Western blot analysis of natural cell products_recover or human body fluids, multiple bands of IL-6 will appear due to the variable amount of glycosylation on the molecule. The antibody is also useful for neutralization of human IL-6 activity in bioassays. For neutralization, incubate the sample with a 1:400 dilution of the antibody for at least 4 hours before testing. A control of similarly diluted normal rabbit IgG (heat inactivated) is recommended. In neutralization experiments in vitro, this antibody does not result in enhanced activity of IL-6. However, because antibodies to IL-6 may act as a soluble receptor in vivo, some antibodies to IL-6 act as carriers and enhance IL-6 activity.

This antibody has been tested for use in neutralizations, ELISA, immunohistochemistry, radioimmunoassay, immunoprecipitation and immunoblotting. It recognizes IL-6. Reactivity in other immunoassays is unknown. For immunoblotting, use the supernatants or lysates of 2 x 106 endotoxin-stimulated human peripheral blood mononuclear cells (PBMC). PBMC are stimulated for 24 hours with 1% (v/v) human plus 10 ng/mL E.coli LPS. For immunohistochemistry, either paraffin fix or cryofix tissue. For immunoprecipitation, preclearing with a non-specific rabbit IgG is helpful to reduce background. For use in ELISA formats, this antibody is best used as the second antibody in combination with a monoclonal antibody as the capture antibody.