Anti-Streptavidin from Streptomyces avidinii

This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material. Contains sodium azide (0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

Product Specifications

Reactivity

Other

Immunogen

Chromatography on protein A Sepharose

Clonality

Monoclonal

Type

Antibodies-Monoclonal

Applications

WB,IHC,ELISA,ICC/IF,Flow cyt

Concentration

1ug/ul

Purity

Chromatography on protein A Sepharose

Buffer

PBS, pH 7.4 with 0.05% sodium azide.

Shipping Conditions

Ice Pack

Storage Conditions

This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Fragment

IgG1, kappa

Specificity

No cross-reactivity with egg white avidin. Affinity (Kd) > 1 x 108 for all MAbs. MAbs bind with high efficiency with different epitopes of streptavidin molecule. MAbs bind to the streptavidin without interfering with the biotin-binding reaction.

Applications Notes

MAbs can be used to localize streptavidin, to amplify signals in immunochemistry, immunohistochemistry, ELISA, flow cytometry and immunoblotting. These mouse anti-streptavidin MAbs applied to the ABC complex, previously bound to the second antibody system allow for further enhancement of signal when anti-mouse antibody conjugate is added. MAbs are effective in immunoadsorbtion procedures of streptavidin - biotinylated protein complexes.