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Anti-NF-kB p65 (Phospho-Thr254)

Product Specifications

CAS Number

9007-83-4

Reactivity

Human,Mouse,Rat

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human NF-κB p65 around the phosphorylation site of threonine 254 (F-R-TP-P-P).

Clonality

Polyclonal

Type

Antibodies-Polyclonal

Applications

WB,IHC

Concentration

1ug/ul

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Buffer

phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Shipping Conditions

Ice Pack

Storage Conditions

This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Specificity

NF-κB p65 (phospho-Thr254) antibody detects endogenous levels of NF-κB p65 only when phosphorylated at threonine 254.

Applications Notes

WB: 1:500~1:1000; IHC: 1:50~1:100

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