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Anti-MARCKS (phospho-Ser170)

The protein encoded by this gene is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis. [provided by RefSeq, Jul 2008]

Product Specifications

Reactivity

Human

Immunogen

The antibody was produced against synthesized phosphopeptide derived from human MARCKS around the phosphorylation site of Serine 170 (G-F-SP-F-K).

Clonality

Polyclonal

Type

Antibodies-Polyclonal

Applications

WB,IHC

Concentration

100ug/100ul

Purity

Affinity purified

Buffer

Rabbit IgG in phosphate buffered saline (without Mg<sup>2</sup>+ and Ca<sup>2</sup>+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Shipping Conditions

Ice Pack

Storage Conditions

This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Fragment

IgG

Specificity

MARCKS (phospho-Ser170) Antibody detects endogenous levels of MARCKS only when phosphorylated at threonine 170.

Applications Notes

IHC: 1:50~100 WB: 1:500~1:1000

CAS Number

9007-83-4

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