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Anti-human IgM

This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material. Contains sodium azide (0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

Product Specifications

CAS Number

9007-83-4

Reactivity

Human

Immunogen

Human serum

Clonality

Monoclonal

Type

Antibodies-Monoclonal

Applications

WB,ELISA,Other

Concentration

1ug/ul

Purity

MAb M11 by chromatography on protein G Sepharose. All other MAbs by chromatography on protein A Sepharose.

Buffer

PBS, pH 7.4 with 0.05% sodium azide.

Shipping Conditions

Ice Pack

Storage Conditions

This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Fragment

IgG1 for MAbs 2A6, 1F4, CH2 and M11 IgG2b for MAbs and MA2

Specificity

Mu-chain of human IgM MAbs 2A6 and CH2 - Fc -region localized epitope MAbs 2B9 and MA2 - Fab - region localized epitope Specific antibody titer is 5 x 10-7 by indirect ELISA.

Applications Notes

Detection of IgM antibodies and total IgM. MAb 2A6 can be used as labeled reagent for IgM or mu-chains detection by means of immunoblotting. MAb 2A6 can be used for passive haemagglutination. MAb 2B9 can be used as labeled reagent for specific IgM antibodies detection. MAb 2B9 can be used also as capture and labeled antibody simultaneously for total IgM level determination by means of ELISA. MAb 1F4 is working in Western blotting.

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