Anti-human IgE

This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material. Contains sodium azide (0.05-0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

Product Specifications

CAS Number

9007-83-4

Reactivity

Human

Immunogen

Myelomatous human serum

Clonality

Monoclonal

Type

Antibodies-Monoclonal

Applications

WB,ELISA

Concentration

1ug/ul

Purity

MAbs 4F4, 5D4, E411, BE5, 4G7 and 4H10 by chromatography on protein A Sepharose MAb XTE4 by ion exchange chromatography

Buffer

PBS, pH 7.4 with 0.05% sodium azide.

Shipping Conditions

Ice Pack

Storage Conditions

This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Fragment

IgG for MAbs BE5, 4G7 and 4H10 IgG1 for MAb XTE4 IgG2a for MAbs 4F4, 5D4 and E411

Specificity

MAb E411 class specific for human IgE, Fc-region specific MAb 4F4 - IgE Cε3-domain specific MAb 5D4 - IgE Cε2-domain specific MAb XTE4 - epsilon chain specific Specific antibody titer is 1/1 000 000 by indirect ELISA.

Applications Notes

Detection of total IgE level and IgE antibodies in ELISA. Recommended pairs (coating - conjugate): 5D4 â€“ 4F4, 4G7â€“ BE5. MAb E411 can be used for coating. MAb 4F4 can be used for IgE purification by affinity chromatography. MAb XTE4 can be used in Western blotting.