Anti-Akt2 (Ab-474)
This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins. [provided by RefSeq, Jul 2008]
Product Specifications
CAS Number
9007-83-4
Reactivity
Human,Mouse,Rat
Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human Akt2 around the phosphorylation site of serine 474 (Q-F-SP-Y-S).
Clonality
Polyclonal
Type
Antibodies-Polyclonal
Applications
WB,IHC
Concentration
1ug/ul
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Buffer
phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Shipping Conditions
Ice Pack
Storage Conditions
Specificity
Akt2 (Ab-474) antibody detects endogenous levels of total Akt2 protein.
Applications Notes
WB: 1:500~1:1000 IHC: 1:50~1:100
Frequently Asked Questions
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