Anti-Rabbit IgG Unlabeled (Chicken Host)
Since chicken IgY molecules are quite different from mammalian IgG’s, our secondary antibodies don’t cross react with primary antibodies made in rabbits, mice, or any other mammalian host tested. This makes it simple to performed double immunostaining.
Product Specifications
CAS Number
9007-83-4
Reactivity
Other
Immunogen
Chickens were immunized with purified, non-denatured rabbit IgG (containing both heavy and light chains). Eggs were collected from hyperimmunized hens and the IgY fraction
purified from the yolks. Anti-rabbit IgG’s were affinity-purified over a column conjugated with purified rabbit IgG (containing both heavy and light chains).
Clonality
Polyclonal
Type
Antibodies-Polyclonal
Applications
WB,ELISA,ICC/IF
Concentration
100ug/100ul
Purity
Affinity purified
Buffer
PBS, pH 7.4 with 0.02% sodium azide.
Shipping Conditions
Ice Pack
Storage Conditions
This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Specificity
Antibodies were tested for specificity using double-immunodiffusion in agarose. There is no detectable cross-reactivity with goat, human or mouse IgG.
Applications Notes
<p>1:1000 Western blots, 1:250 Immunohistochemistry.</p>
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