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Anti-Rabbit IgG Unlabeled (Chicken Host)

Since chicken IgY molecules are quite different from mammalian IgG’s, our secondary antibodies don’t cross react with primary antibodies made in rabbits, mice, or any other mammalian host tested. This makes it simple to performed double immunostaining.

Product Specifications

CAS Number

9007-83-4

Reactivity

Other

Immunogen

Chickens were immunized with purified, non-denatured rabbit IgG (containing both heavy and light chains). Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-rabbit IgG’s were affinity-purified over a column conjugated with purified rabbit IgG (containing both heavy and light chains).

Clonality

Polyclonal

Type

Antibodies-Polyclonal

Applications

WB,ELISA,ICC/IF

Concentration

100ug/100ul

Purity

Affinity purified

Buffer

PBS, pH 7.4 with 0.02% sodium azide.

Shipping Conditions

Ice Pack

Storage Conditions

This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Specificity

Antibodies were tested for specificity using double-immunodiffusion in agarose. There is no detectable cross-reactivity with goat, human or mouse IgG.

Applications Notes

<p>1:1000 Western blots, 1:250 Immunohistochemistry.</p>

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