Recombinant Rat TNF
TNF is secreted by macrophages, monocytes, neutrophils, T cells, natural killer cells following their stimulation by bacterial lipopolysaccharides. Cells expressing CD4 secrete TNF-alpha while CD8(+) cells secrete little or no TNF-alpha. Stimulated peripheral neutrophilic granulocytes but also unstimulated cells and also a number of transformed cell lines, astrocytes, microglial cells, smooth muscle cells, and fibroblasts also secrete TNF. Human milk also contains this factor. The synthesis of TNF-alpha is induced by many different stimuli including interferons, IL-2, GM-CSF, SP, Bradykinin, Immune complexes, inhibitors of cyclooxygenase and PAF (platelet activating factor). Human TNF-alpha is a non-glycosylated protein of 17 kDa and a length of 157 amino acids. Murine TNF-alpha is N-glycosylated. Homology with TNF-beta is approximately 30%. TNF-alpha forms dimers and trimers.
Product Specifications
Gene Name
TNF
Gene Aliases
Gene ID
24835
Accession Number
P16599
Organism
Rat
Source
E. coli
Concentration
<1.0 EU/μg of recombinant protein as determined by the LAL method
Purity
>95% as determined by SDS-PAGE
Solubility
Function
The ED50 as determined by the dose-dependent stimulation of the proliferation of monkey 4MBr-5 cells was found to be in the range of 20-40 ng/mL
Molecular Weight
17
Shipping Conditions
Ambient Temperature
Storage Conditions
Appearance
Lyophilized Powder
Formulation
Lyophilized from a 0.2 μm filtered PBS solution (pH 7.0)
CAS Number
9000-83-3
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