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Recombinant Rat Eotaxin (CCL11)

Eotaxin belongs to the platelet factor-4 family of chemokines. The gene symbol is SCYA11. This factor is referred to also as Eotaxin-1 and has been renamed CCL11. Eotaxin induces substantial accumulation of eosinophils in the skin without significantly affecting the accumulation of neutrophils. Eotaxin is a potent stimulator of both guinea pig and human eosinophils in vitro. The Eotaxin receptor is a G-protein-coupled receptor selectively expressed in human eosinophils.

Product Specifications

Gene Name

CCL11

Gene Aliases

C-C motif chemokine 11, Eosinophil chemotactic protein, Small-inducible cytokine A11, Scya11

Gene ID

29397

Accession Number

P97545

Organism

Rat

Source

E. coli

Concentration

<1.0 EU/μg of recombinant protein as determined by the LAL method

Purity

>95% as determined by SDS-PAGE

Solubility

A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Function

Activity was determined by the ability to chemoattract human peripheral blood eosinophils using a concentration range of 0.1-20 ng/mL.

Molecular Weight

8

Shipping Conditions

Ambient Temperature

Storage Conditions

The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Appearance

Lyophilized Powder

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS

CAS Number

9000-83-3

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