What is the catalog number for Recombinant Mouse TNF?

The catalog number for Recombinant Mouse TNF is Z200215.

What sizes are available for Recombinant Mouse TNF?

Recombinant Mouse TNF is available in the following size(s): 50 µg.

Is Recombinant Mouse TNF in stock?

Recombinant Mouse TNF is currently made to order / not held in immediate stock. Contact us for lead time.

Recombinant Mouse TNF

TNF is secreted by macrophages, monocytes, neutrophils, T cells, natural killer cells following their stimulation by bacterial lipopolysaccharides. Cells expressing CD4 secrete TNF-alpha while CD8(+) cells secrete little or no TNF-alpha. Stimulated peripheral neutrophilic granulocytes but also unstimulated cells and also a number of transformed cell lines, astrocytes, microglial cells, smooth muscle cells, and fibroblasts also secrete TNF. Human milk also contains this factor. The synthesis of TNF-alpha is induced by many different stimuli including interferons, IL-2, GM-CSF, SP, Bradykinin, immune complexes, inhibitors of cyclooxygenase and PAF (platelet activating factor). Human TNF-alpha is a non-glycosylated protein of 17 kDa and a length of 157 amino acids. Murine TNF-alpha is N-glycosylated. Homology with TNF-beta is approximately 30%. TNF-alpha forms dimers and trimers.

Product Specifications

CAS Number

9000-83-3

Gene Name

TNF

Gene Aliases

TNF-alpha, TNF-a, Tumor necrosis factor ligand superfamily member 2, Cachectin, Tnfa, Tnfsf2, Tnf<br/>Recombinant Mouse Tumor Necrosis Factor Alpha (TNF)

Gene ID

21926

Accession Number

P06804

Organism

Mouse

Source

E. coli

Concentration

<1.0 EU/μg of recombinant protein as determined by the LAL method.

Purity

>95% as determined by SDS-PAGE

Solubility

A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Function

The ED(50) was determined cytolysis of murine L929 cells in the presence of Actinomycin D is ≤ 0.05 ng/mL, corresponding to a specific activity of ≥ 3.0 x 10^7 units/mg.

Molecular Weight

17

Shipping Conditions

Ambient Temperature

Storage Conditions

The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.