Recombinant Mouse IL25

IL-25 (also known as IL- 17E), a member of the IL-17 family, has been implicated in Th2 cell-mediated immunity. Recently, IL-25 has been identified as one of the initiators of the Th2 response (10, 11) and has also been shown to be expressed by mast cells. The transgenic overexpression of IL-25 by lung epithelial cells results in mucus hyperproduction and airway infiltration of macrophages and eosinophils; conversely, IL-25 blockade reduced airway inflammation and Th2 cytokine production in an allergen-induced asthma model.

Product Specifications

CAS Number

9000-83-3

Gene Name

IL25

Gene Aliases

UPF0556 protein, IL-25, Stromal cell-derived growth factor SF20, D17Wsu104e, Il25<br/>Recombinant Mouse Interleukin-25 (IL25)

Gene ID

140806

Accession Number

Q9CPT4

Organism

Mouse

Source

E. coli

Concentration

<1.0 EU/μg of recombinant protein as determined by the LAL method

Purity

>95% as determined by SDS-PAGE

Solubility

A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Function

The ED(50) was determined by the dose-dependent proliferation of human umbilical vein endothelial cells and was found to be <0.5ng/mL.

Molecular Weight

Shipping Conditions

Ambient Temperature

Storage Conditions

Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.