Recombinant Human VEGF (121aa)

VEGF is a heavily glycosylated, homodimeric protein. The human factor occurs in several molecular variants of 121, 162, 145, 148, 165, 183, 189, 206 amino acids, arising by alternative splicing of the mRNA. The splice forms of VEGF differ in biological properties such as the receptor types, which they recognize and their interaction with heparan sulfate proteoglycans. VEGF121 and VEGF165 are soluble secreted forms of the factor. The interaction of VEGF with heparin-like molecules of the extracellular matrix is required for efficient receptor binding. Protamine sulfate and suramin are capable of replacing the receptor-bound factor. This recombinant construct represents the isoform 121 of VEGFA.

Product Specifications

CAS Number

9000-83-3

Gene Name

VEGFA

Gene Aliases

Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF-121aa, Recombinant Human Vascular Endothelial Growth Factor 121AA (VEGFA)

Gene ID

7422

Accession Number

P15693

Organism

Human

Source

E. coli

Concentration

< 1.0 EU/μg of recombinant protein as determined by the LAL method.

Purity

>95% as determined by SDS-PAGE

Solubility

A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Function

The ED50 as determined by the dose-dependent proliferation of human umbilical vein endothelial cells (HUVEC) was found to be 10 ng/mL

Molecular Weight

14.3

Shipping Conditions

Ambient Temperature

Storage Conditions

The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.