Recombinant Human TNF
Tumor Necrosis Factor is secreted by macrophages, monocytes, neutrophils, T cells, natural killer cells following their stimulation by bacterial lipopolysaccharides although cells expressing CD8(+) secrete little or no TNF-alpha. In addition, TNF is secreted by peripheral neutrophilic granulocytes and by a number of transformed cell lines that include those of astrocytes, microglial cells, smooth muscle cells and fibroblasts. Human milk also contains this factor. The synthesis of TNF-alpha is induced by many different stimuli including interferons, IL2, GM-CSF, SP, Bradykinin, Immune complexes, inhibitors of cyclooxygenase and platelet activating factor. TNF-alpha shares approximately 30% homology with TNF-beta. Human TNF-alpha is a non-glycosylated protein of 17.6 kDa.
Product Specifications
CAS Number
9000-83-3
Gene Name
TNF
Gene Aliases
TNFSF2, Cachectin, Differentiation-inducing factor (DIF), Necrosin, Cytotoxin<br/>Recombinant Human Tumor Necrosis Factor Alpha (TNF)
Gene ID
7124
Accession Number
P01375
Organism
Human
Source
E. coli
Concentration
<1.0 EU/μg of recombinant protein as determined by the LAL method
Purity
>95% as determined by SDS-PAGE
Solubility
Function
The ED50 as determined by dose dependent proliferation of IL4 stimulated human megakaryocytic cell line was found to be ≤0.5 ng/mL
Molecular Weight
17.6
Shipping Conditions
Ambient Temperature
Storage Conditions
Appearance
Lyophilized Powder
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS (pH 7.2)
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