Recombinant Human TGFA

An EGF family member, TGF-A is a cytokine that is produced following the protease digestion of its trans-membrane precursor proTGF-A. TGF-A is expressed both in normal tissues during embryogenesis and in adult tissues, such as the brain, keratinocytes, and macrophages and at high levels in transformed and tumour cells.

Product Specifications

CAS Number

9000-83-3

Gene Name

TGFA

Gene Aliases

TGF-alpha, TGF-A, isoform CRA_d<br/>Recombinant Human Transforming Growth Factor Alpha (TGFA)

Gene ID

7039

Accession Number

P01135

Organism

Human

Source

E. coli

Concentration

<1.0 EU/μg of recombinant protein as determined by the LAL method.

Purity

>95% as determined by SDS-PAGE

Solubility

Reconstitute at 100 μg/mL in sterile 10 mM Acetic Acid

Function

Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED(50) for this effect is typically 0.10.4 ng/mL.

Molecular Weight

5.8

Shipping Conditions

Ambient Temperature

Storage Conditions

The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.