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Recombinant Human PTH

Parathyroid hormone is a circulating hormone that acts as the central regulator of calcium metabolism by directly targeting bone, kidney, and intestine. The classical concept of PTH action is that it regulates serum calcium levels by stimulating bone resorption. However, intermittent administration of PTH selectively stimulates bone formation. It is now known that PTH binds to its receptor PTH1R, and activates the G protein alpha subunits. Besides PKA and PKC activation, PTH also regulates MAPKs including p42/p44 ERKs, p38, and c-Jun N-terminal kinase subtypes.

Product Specifications

Gene Name

PTH

Gene Aliases

PTH, Parathormone, Parathyrin<br/>Recombinant Human Parathyroid Hormone (PTH)

Gene ID

5741

Accession Number

P01270

Organism

Human

Source

E. coli

Concentration

<1.0 EU/μg of recombinant protein as determined by the LAL method

Purity

>95% as determined by SDS-PAGE

Solubility

A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Function

The ED(50) was determined by the dose-dependent proliferation of Immortalized Human Osteoblasts- SV40 and was found to be &lt;0.5ng/mL.

Molecular Weight

9

Shipping Conditions

Ambient Temperature

Storage Conditions

The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Appearance

Lyophilized Powder

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS

CAS Number

9000-83-3

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