Recombinant Human Nodal

Nodal, also known as Nodal homolog, is a secreted protein member of the Transforming Growth Factor-beta (TGF-beta) superfamily. It is essential for the formation of mesoderm and axial patterning during early vertebrate embryonic development.

Product Specifications

Gene Name

NODAL

Gene Aliases

Nodal homolog

Gene ID

4838

Accession Number

Q96S42

Organism

Human

Source

E. coli

Concentration

<1.0 EU/μg of recombinant protein as determined by the LAL method

Purity

>95% as determined by SDS-PAGE

Solubility

Reconstitute in sterile 4mM HCl at 100µg/mL (containing at least 0.1% human or bovine serum albumin)

Function

100 ng/mL phosphorylation activity as determined by its ability to induce Smad2 phosphorylation in P19 mouse embryonal carcinoma cells

Molecular Weight

13.1

Shipping Conditions

Ambient Temperature

Storage Conditions

The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Appearance

Lyophilized Powder

Formulation

Lyophilized from a 0.2 µm filtered solution in Acetonitrile and TFA (with BSA as a carrier protein)

CAS Number

9000-83-3

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