Recombinant Human MIP-4 (CCL18)

CCL18, also designated dendritic cell (DC)3-derived CC chemokine 1, pulmonary and activation-regulated chemokine, alternative macrophage activation-associated CC chemokine 1, and MIP-4, is a human chemokine structurally related to CCL3. CCL18 was shown to be expressed in germinal centers of tonsils by dendritic cells and to attract mainly naive T cells, CD38 mantle zone B lymphocytes and DC. The production of CCL18 by APC is enhanced by Th2 cytokines like IL-4 and IL-13 and suppressed by IFN-gamma.

Product Specifications

CAS Number

9000-83-3

Gene Name

CCL18

Gene Aliases

MIP4, CCL18, Pulmonary and activation-regulated chemokine, PARC, DC-CK1, AMAC-1, C-C motif chemokine 18, Small-inducible cytokine A18<br/>Recombinant Human Macrophage Inflammatory Protein-4 (CCL18)

Gene ID

6362

Accession Number

P55774

Organism

Human

Source

E. coli

Concentration

<1.0 EU/μg of recombinant protein as determined by the LAL method.

Purity

>95% as determined by SDS-PAGE

Solubility

A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Function

Determined by its ability to chemoattract human T cells using a concentration range of 2-40 ng/mL.

Molecular Weight

Shipping Conditions

Ambient Temperature

Storage Conditions

The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.