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Recombinant Human MIG (CXCL9)

MIG (CXCL9) protein belongs to the family of chemotactic cytokines known as chemokines. The synthesis of MIG is specifically induced in macrophages and in other cells by IFN-gamma.Human neutrophils produce mig in response to IFN-gamma in combination with either TNF-alpha or bacterial lipopolysaccharides and this response is blocked by IL-10 and IL-4. MIG is a chemoattractant for stimulated but not resting T cells, however it is not active on neutrophils or monocytes.

Product Specifications

CAS Number

9000-83-3

Gene Name

CXCL9

Gene Aliases

C-X-C motif chemokine 9, Gamma-interferon-induced monokine, Monokine induced by interferon-gamma, Small-inducible cytokine B9, CMK, HuMIG, SCYB9, CXCL9

Gene ID

4283

Accession Number

Q07325

Organism

Human

Source

E. coli

Concentration

<1.0 EU/μg of recombinant protein as determined by the LAL method.

Purity

>95% as determined by SDS-PAGE

Solubility

A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Function

Determined by its ability to chemoattract human peripheral blood T lymphocytes using a concentration range of 10-80 ng/mL.

Molecular Weight

11

Shipping Conditions

Ambient Temperature

Storage Conditions

The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Appearance

Lyophilized Powder

Formulation

Recombinant MIG/CXCL9 was lyophilized from 1.0 mg/mL solution in 25 mM NaCl 10 mM PB pH 7.0.

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