Recombinant Human MANF

MANF is a neurotrophic factor found mainly in central nervous tissues where it may remain localized to the endoplasmic reticulum/Golgi apparatus, or secreted. It has been shown that MANF plays an important role in protecting cells against tunicamycin and thapsigargin -induced cell death. Without MANF, cells become increasingly vulnerable to the aforementioned drugs. Also, MANF is structurally homologous to CDNF.

Product Specifications

CAS Number

9000-83-3

Gene Name

MANF

Gene Aliases

Arginine-rich protein, Protein ARMET, ARP<br/>Recombinant Human Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF)

Gene ID

7873

Accession Number

P55145

Organism

Human

Source

E. coli

Concentration

<1.0 EU/μg of recombinant protein as determined by the LAL method

Purity

>95% as determined by SDS-PAGE

Solubility

Reconstitute at 200 μg/mL in PBS

Function

The ED50 was found to be <0.5ng/ml using dose dependent proliferation of human pancreatic cells.

Molecular Weight

18.4

Shipping Conditions

Ambient Temperature

Storage Conditions

The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.