Recombinant Human LIF

LIF is a multifunctional secreted glycoprotein that exists in both soluble and matrix bound forms. It displays biologic activities ranging from the differentiation of myeloid leukemic cells into macrophage lineage to effects on bone metabolism, inflammation, neural development, embryogenesis, and the maintenance of implantation. It is now clear that LIF is related in both structure and mechanism of action to the interleukin IL-6 family of cytokines, which also includes IL-11, ciliary neurotrophic factor, oncostatin M, and cardiotrophin 1. The actions of these cytokines are mediated through specific cell-surface receptors that consist of a unique chain and the shared signal transducing subunit gp130.

Product Specifications

CAS Number

9000-83-3

Gene Name

LIF

Gene Aliases

LIF, Differentiation-stimulating factor, D factor, Melanoma-derived LPL inhibitor (MLPLI) <br/>Recombinant Human Leukemia Inhibitory Factor (LIF)

Gene ID

3976

Accession Number

P15018

Organism

Human

Source

E. coli

Concentration

<1.0 EU/μg of recombinant protein as determined by the LAL method

Purity

>95% as determined by SDS-PAGE

Solubility

A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Function

The ED50 was in found to be <0.2 ng/ml as determined by the dose-dependent proliferation of human erythroleukemic cell line.

Molecular Weight

Shipping Conditions

Ambient Temperature

Storage Conditions

The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.