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Recombinant Human Leptin (LEP)

Leptin is a cytokine found in the adipose tissue of animals (Leptin mRNA is restricted to the adipose tissue). Leptin has been shown to suppress appetite, increase thermogenesis and reduce body weight, food consumption and plasma glucose levels.

Product Specifications

CAS Number

9000-83-3

Gene Name

LEP

Gene Aliases

OB, Obese protein, Obesity factor

Gene ID

3952

Accession Number

P41159

Organism

Human

Source

E. coli

Concentration

<1.0 EU/μg of recombinant protein as determined by the LAL method

Purity

>95% as determined by SDS-PAGE

Solubility

Reconstitute at 1 mg/mL in sterile 20 mM Tris-HCI (pH 8.0).

Function

The ED(50) was determined by the dose-dependent proliferation of human umbilical vein endothelial cells and was found to be <0.5ng/mL.

Molecular Weight

16.4

Shipping Conditions

Ambient Temperature

Storage Conditions

The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Appearance

Lyophilized Powder

Formulation

Lyophilized from a 0.2 μm filtered solution in Tris-HCl and NaCl.

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