Recombinant Human IL2

A helical bundle of 4 α-helices, IL2 is an immunoregulatory cytokine that is expressed by CD4+/CD8+ T cells, γδ T cells, B cells, eosinophils and dendritic cells. IL2 stimulates growth and differentiation of B-cells, NK cells, lymphokine-activated killer cells, monocytes, macrophages and oligodendrocytes. While Human IL2 shares 56% and 66% amino acid sequence identity with mouse and rat IL2, respectively only human and mouse IL2 exhibit cross-species activity. Characteristically, IL2 exerts both autocrine and paracrine activity on T cells. In a regulatory role, IL2 is important for the development, survival, and function of regulatory T cells, it enhances Fas-mediated activation-induced cell death, and it inhibits the development of inflammatory Th17 cells. Recombinant Human IL2 is a non-glycosylated polypeptide that contains one intrachain disulfide bond.

Product Specifications

CAS Number

9000-83-3

Gene Name

IL2

Gene Aliases

IL-2, T-cell growth factor, TCGF, Aldesleukin, INN=Aldesleukin<br/>Recombinant Human Interleukin-2 (IL2)

Gene ID

3558

Accession Number

P60568

Organism

Human

Source

E. coli

Concentration

<1.0 EU/μg of recombinant protein as determined by the LAL method.

Purity

>95% as determined by SDS-PAGE

Solubility

A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Function

The ED50 as determined by the dose dependent proliferation of human T cells was found to be less than 0.5 ng/mL

Molecular Weight

Shipping Conditions

Ambient Temperature

Storage Conditions

The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.