Recombinant Human IL17F

Interleukin 17F is a disulfide-linked homodimeric protein belonging to the IL-17 family of proinflammatory cytokines. Members belonging to this family share conserved features of cysteine-knot structures but differ in their N-terminal regions. The T-helper cell produced IL-17 family is inducible by IL15 and IL23, and IL17F induces T-cell and PBMC activation and proliferation. It also performs cartilage matrix turnover and angiogenesis prevention. Out of the IL-17 family, IL17F is the most homologous to IL17 and dimerizes in a way similar to that of nerve growth factors (NGF) and other neurotrophins.

Product Specifications

CAS Number

9000-83-3

Gene Name

IL17F

Gene Aliases

Cytokine ML-1

Gene ID

112744

Accession Number

Q96PD4

Organism

Human

Source

E. coli

Concentration

<1.0 EU/μg of recombinant protein as determined by the LAL method

Purity

>95% as determined by SDS-PAGE

Solubility

A quick spin of the vial followed by reconstitution in sterile distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Function

The ED50 is 8.0-40.0ng/mL as determined by its ability to induce IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells

Molecular Weight

15.2

Shipping Conditions

Ambient Temperature

Storage Conditions

The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.